Plant Tissue Culture And Transformation Techniques

This publication deals with various aspects of the genetic engineering-plant tissue culture and transformation techniques. Due to their biological, ecological and geographic diversity, the demand for various horticultural crops is likely to increase manifold in the future and in order to meet such demand, there is an urgent need to concentrate on the research aspects for improvement of these crops. Plant tissues culture offers new tools to accomplish this objective. Plant tissue culture is an important area of biotechnology, whic is used for the propagation of problem-species, rapid propagation of high value genotypes, production of secondary metabolites etc. Tissue culture is an important step in developing new hybrids from distant parents and transgenics and particularly cost-effective technology with palpable impact in vegetatively propagated plants, which is celarly visible in improved yields of cultivars incorporating genes from unexplored sources and improved germplasm, enhancement of quality parameters and supply of disease-free clones of true-to-type planting materials. Plant tissue culture is the most rapid and efficacious way to speedy productin of large volumes of identical plants for specific markets. Micropropagation is the quickest way for popularization of new varieties of horiticultural crops where other methods of mass multiplication of genetically pure and homogeneous planting materials are very slow. With the advent of transformation technology, it has become a useful tool to mass produce new plants with genetic material transferred from unrelated sources with the help of tissue culture. The volume contains contributions by several authors highlighting the status of genetic engineering and plant tissue culture research and development programmes in various developing countries and case studies on a few economically important crops. The publication will be of immense value to the working scientists, institutions, policy makers and all those bearing responsibility to develop, implement and intensify programmes in the related subjects in their respective countries. This book provides a good picture of efforts being made and success already achieved in the Third World countries at various levels of development striving to secure gains from the latest advances in science and technology. Contents Chapter 1: China-Cotton Genetic Engineering and Tissue Culture Developments by Reddy Naganagouda and Zhu Shuijin; Chapter 2: Egypt: Development of Transgenic Wheat with Improved Salt and Drought Tolerance by Ahmed Bahelidin & Hala F Eissa; Chapter 3: Egypt-Use of Genetic Engineering Approach to Develop Virus Resistance for Some Plants Belonging to Different Plant Families by Atef Shoukry Sadik; Chapter 4: Egypt-Genetic Transformation of Maize (Zea mays L) by Shireen Assem; Chapter 5: Egypt-Tissue Culture and Transformation of Potato by Taymour Nasr El Din; Chapter 6: Eritrea-Genetic Engineering by Tadesse Mehari; Chapter 7: India-Present Status, Policy and Constrains in Genetic Engineering by Jeetendra Jaysing Solanki; Chapter 8: Indonesia-Review on the Role of Biotechnology for Food Security by Lukit Devy; Chapter 9: Iran-Status of Agricultural Biotechnology by M Kafi; Chapter 10: Kenya-Status of Biotechnology Research and Development by C N Ngaman, M G Karembu and D Otunge; Chapter 11: Kenya-Present Status, Policies and Constraints in Areas Related to Plant Biotechnology by Salome Mallowa Obura; Chapter 12: Malaysia-A Brief Report on Biotechnology and Genetic Engineering by Z A Aziz; Chapter 13: Pakistan-Present Status, Policies and Constraints of Biotechnology by Saghir Ahmed Sheikh; Chapter 14: Sri Lanks-Present Status of Biotechnology by P Aruni Weerasinghe; Chapter 15: Syria-Current Status and Future Prospective of Agricultural Biotechnology Program at GCSAR by Nabila Ali Bacha; Chapter 16: Uganda-Report on the Present Status Policies and Constraints to Genetic Engineering by Kyeyune Gerald Muwanga.

Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, addresses the most current principles and methods in plant tissue culture research. The editors use the expertise of some of the top researchers and educators in plant biotechnology to furnish students, instructors and researchers with a broad consideration of the field. Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. New topic discussions and laboratory exercises in the Second Edition include ""Micropropagation of Dieffenbachia,"" ""Micropropagation and in vitro flowering of rose,"" ""Propagation from nonmeristematic tissue-organogenesis,"" ""Variation in culture"" and ""Tissue culture of ferns.""It is the book's extensive laboratory exercises that provide a hands-on approach in illustrating various topics of discussion, featuring step-by-step procedures, anticipated results, and a list of materials needed. What's more, editors Trigiano and Gray go beyond mere basic principles of plant tissue culture by including chapters on genetic transformation techniques, and photographic methods and statistical analysis of data. In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, is a veritable harvest of information for the continued study and research in plant tissue culture science.

This manual comprises a range of techniques for research workers in the fields of cell and molecular biology, physiology, plant breeding and propagation, and genetic engineering.

Plant Cell and Tissue Culture gives an exhaustive account of plant cell culture and genetic transformation, including detailed chapters on all major field and plantation crops. Part A presents a comprehensive coverage of all necessary laboratory techniques for the initiation, nutrition, maintenance and storage of plant cell and tissue cultures, including discussions on these topics, as well as on morphogenesis and regeneration, meristem and shoot tip culture, plant protoplasts, mutant cell lines, variation in tissue cultures, isogenic lines, fertilization control, cryopreservation, transformation, and the production of secondary metabolites. Part B then proceeds into detail on the specific in vitro culture of specific crops, including cereals, legumes, vegetables, potatoes, other roots and tubers, oilseeds, temperate fruits, tropical fruits, plantation crops, forest trees and ornamentals. Plant Cell and Tissue Culture is, and is likely to remain, the laboratory manual of choice, as well as a source of inspiration and a guide to all workers in the field.

Plant Tissue Culture forms an integral basis of the present day biotechnology. Plant Tissue Culture: Practices and New Experimental Protocols is being brought out to fill the existing gap in the available literature on plant tissue culture, especially focusing on the aspects of practical procedures and protocols of tissue culture. This book contains important experimental techniques and gives guidance on carrying out hands-on experiences. It has been designed in a simple way, giving all the necessary procedures as a general guideline and also necessary tips to maneuver any problem encountered. These tips are based on the first hand experiences of the author while teaching and researching the techniques of plant tissue culture. A unique feature of this book is the inclusion of several techniques describing the actual protocols experimented and developed with different plant species by different scientists. A substantial number of original colored plates including fluorescence photographs standout the book. This pioneering work is valuable for the students who are looking for fresh outlook and search.

Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature

Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnol

During the past decade, Plant Tissue Culture (PTC) has attracted considerable attention because of its vital role in plant biotechnology. PTC offers novel approaches to plant production, propagation, and preservation. Some in vitro techniques are being applied on a commercial scale while many others hold great potential. Consequently, the literature in this area has grown rapidly.This book deals with recent developments in plant tissue culture, and presents a critical assessment of the proven and potential applications of the various in vitro techniques, it also highlights current problems limiting the application of tissue culture, and projects the future lines of research in this field.