Polymers And Nanomaterials For Gene Therapy

Polymers and Nanomaterials for Gene Therapy provides the latest information on gene therapy, a topic that has attracted significant attention over the past two decades for the treatment of inherited and acquired genetic diseases. Major research efforts are currently focused on designing suitable carrier vectors that compact and protect oligonucleotides for gene therapy. The book explores the most recent developments in the field of polymer science and nanotechnology, and how these advancements have helped in the design of advanced materials. Non-viral vector systems, including cationic lipids, polymers, dendrimers, peptides and nanoparticles, are potential routes for compacting DNA for systemic delivery. However, unlike viral analogues that have no difficulty in overcoming cellular barriers and immune defense mechanisms, non-viral gene carriers consistently exhibit significant reduced transfection efficiency due to numerous extra- and intracellular obstacles. Therefore, biocompatibility and potential for large-scale production make these compounds increasingly attractive for gene therapy. This book contains chapters on the engineering of polymers and nanomaterials for gene therapy, and how they can form complexes with DNA and avoid both in vitro and in vivo barriers. Other chapters describe in vitro, ex vivo, in vivo gene therapy studies, and the current issues affecting non-viral gene therapy. Explores current challenges in the research of genetic diseases Discusses polymers for gene therapy and their function in designing advanced materials Provides examples of organic and inorganic nanomaterials for gene therapy Includes labeling, targeting, and assays Looks at characterization, physico-(bio)chemical properties, and applications

To treat disease or correct genetic disorders using gene therapy, the most suitable vehicle must be able to deliver genes to the appropriate tissues and cells in the body in a specific as well as safe and effective manner. While viruses are the most popular vehicles to date, their disadvantages include toxicity, limited size of genes they can carry

Polymeric Nanoparticles as Promising Tool for Anti-cancer Therapeutics provides an understanding of polymeric compounds and their use in cancer therapies. The book begins by giving an overview of the current status, future challenges and potential utilization of polymeric nanoparticles. It then covers specific polymeric nanoparticles through contributions from world-renowned experts and researchers. Chapters examine specific polymeric nanoparticles, their development as potential targeted delivery systems, and cancer characteristics that can be targeted for therapy development. The book synthesizes current research trends in the field, thus enhancing existing knowledge of nanomedicine, drug delivery and therapeutic intervention strategies in human cancers. Users will find this to be an ideal reference for research scientists and those in the pharmaceutical and medical fields who are working to develop novel cancer therapies using nanoparticle-based delivery systems. Explores the development of polymeric nanoparticle systems for the purpose of cancer therapy Presents thoroughly analyzed data and results regarding the usage of polymeric nanoparticles-based platforms for the diagnosis and treatment of cancer Highlights various cancer characteristics that can be targeted for therapeutic development using polymeric nanoparticles

​The series Topics in Current Chemistry Collections presents critical reviews from the journal Topics in Current Chemistry organized in topical volumes. The scope of coverage is all areas of chemical science including the interfaces with related disciplines such as biology, medicine and materials science. The goal of each thematic volume is to give the non-specialist reader, whether in academia or industry, a comprehensive insight into an area where new research is emerging which is of interest to a larger scientific audience. Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years are presented using selected examples to illustrate the principles discussed. The coverage is not intended to be an exhaustive summary of the field or include large quantities of data, but should rather be conceptual, concentrating on the methodological thinking that will allow the non-specialist reader to understand the information presented. Contributions also offer an outlook on potential future developments in the field. The chapter "Polymeric Nanoparticle-Mediated Gene Delivery for Lung Cancer Treatment" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Gene therapy is emerging as a new class of therapeutics for the treatment of inherited and acquired diseases. However, poor cellular uptake and instability of DNA in the physiological milieu limits its therapeutic potential, hence a vector which can protect and efficiently transport DNA to the target cells must be developed. Nanotechnology-based non-viral vectors have been proposed as potential candidates. Various polymeric nanoparticles have been shown to be suitable, with high cellular uptake efficiencies and reduced cytotoxicity. These delivery vectors form condensed complexes with DNA which result in shielding against enzymatic degradation and enhanced cellular targeting. Advantages including easy manipulatibility, high stability, low cost and high payload, mean that nanoparticles from various polymers have been exploited. Gene therapy gives a systematic account of the many aspects of nanotechnology mediated gene therapy, from the preparation of nanoparticles to physicochemical characterization, and follows with applications in in vitro and in vivo models. This book emphasizes the various aspects of nanotechnology-based gene therapy, with initial chapters detailing the tools and techniques available for preparation and in vitro and in vivo characterization of nanoparticles. Later chapters provide exhaustive details on polymeric systems employed for gene therapy. Provides an overview of nanotechnology applications in gene therapy, from preparation of nanoparticles to in vitro and in vivo studies Details the tools and techniques available for preparation, characterization and in vitro and in vivo study of nanoparticles Details the limitations of nanoparticle-mediated gene therapy and proposes ways in which they may be overcome

This book focuses on various aspects of nanotechnology based gene silencing. The initial chapters detail the techniques available for in vitro and in vivo characterization of nanoparticles. In the later chapters, exhaustive details about various polymeric systems employed for gene silencing has been accounted.

The field of genetics is rapidly evolving, and new medical breakthroughs are occurring as a result of advances in our knowledge of genetics. Advances in Genetics continually publishes important reviews of the broadest interest to geneticists and their colleagues in affiliated disciplines. Includes methods for testing with ethical, legal, and social implications Critically analyzes future directions Written and edited by recognized leaders in the field

This book intends to provide an up-to-date information in the field of nanobiomedicine. The focus of the book is on the basic concepts and recent developments in the field of nanotechnology. This book covers a broad spectrum of nanomaterials processing, structural characteristics, and related properties and will include bio-probes, medical imaging, drug delivery, and tumor diagnosis. Critical issues are addressed in a straightforward manner so those with no technical background and university students can benefit from the information. Furthermore, many novel concepts in nanomaterials are explained in light of current theories. An important aspect of the book lies on its wide coverage in practical biomedical applications. Not only are the cutting-edge technologies in modern medicine introduced, but also unique materials applications in many clinical areas. Contents:Up-Conversion Nanoparticles for Early Cancer Diagnosis (Liang Cheng, Bingbo Zhang and Zhuang Liu)Redox-Sensitive Polymeric Nanoparticles for Intracellular Drug Delivery (Huiyun Wen, Yongyong Li and Xin Zhao)Design of Biocompatible PEIs as Artificial Gene Delivery Vectors (Ting Shi, Chao Lin and Peng Zhao)Magnetic Fe3O4 Nanoparticles for Cancer Photothermal Therapy (Maoquan Chu)Nanomaterial-Based Sensors for Environmental Monitoring (Jinhu Yang)Janus Nanostructures and Their Bio-Medical Applications (Yilong Wang and Feng Wang)Nanomaterial-Involved Optical Imaging/Spectroscopy Methods for Single-Molecule Detections in Biomedicine (Yao Qin)Surface Functionalized Carbon Nanotubes for Biomedical Applications (Bingdi Chen)Intracellular siRNA Delivery by Multifunctional Nanoparticles (Kun Lu, Lei Wang, Xiaohui Tan, Yao Chen, Changhui Wang and Lifeng Qi)Chemical and Biological Sensors Based on Nanowire Transistors (Xiaohan Wu and Jia Huang) Readership: Undergraduate or graduate students, and researchers in nanomaterials, biochemistry, biomedical engineering. Key Features:It is closely clinically oriented in medical diagnosis and treatmentIt is comprehensive in both nanomaterials and biomedical applicationsIt includes many novel concepts in nanotechnology and up to dateKeywords:Nanomedicine;Nanobiomedicine;Nanobiotechnology;Nanomaterials;Bio-Probes;Medical Imaging;Drug delivery;Tumor Diagnosis