Practical Applications Of Chlorophyll Fluorescence In Plant Biology

The technique of chlorophyll fluorescence has a relatively short history, beginning with the observations by Kautsky (Kautsky and Hirsch, 1931). Since that time there have been several· reviews devoted to the subject, with most of them highly theoretical (Bohlar-Nordenkampf and Oquist, 1993; Dau, 1994; Schreiber et aI. , 1994). There have also been many books devoted to generalized spectrophotometric and microscopic fluorescence techniques. However, to the best of our knowledge there has not been a book completely devoted to the practical applications and uses of chlorophyll fluorescence in plant biology. As techniques mature, applications multiply and so do their potential advantages. The chlorophyll fluorescence technique is maturing as can be seen in the increasing numbers of publications that are devoted to its use. Therefore, we considered that now was a good time to compile the existing knowledge for the applied use of this technique and provide a single volume to which a novice or experienced user could refer. Highly trained experts in the field of photobiology have primarily used the chlorophyll fluorescence technique in the past. In that work, understanding the mechanisms and controls of the photosynthetic processes was the main focus of activity and discussion. Much of the equipment used was highly specialized and expensive, or in some cases one-of-a-kind lab designed units. However, the development of several reliable commercially available chlorophyll fluorescence monitoring instruments has changed the potential user base for the technique.

Chlorophyll a fluorescence is a tool for evaluating plant responses to stress conditions. Fluorescence can be used in plant phenotyping and breeding programs to monitor biotic and abiotic stresses including mineral deficiencies, soil salinity, and pathogenic diseases. Chlorophyll Fluorescence: Understanding Crop Performance — Basics and Applications reviews a diversity of instruments available for recording and analyzing different types of light signals from plants and addresses the use of chlorophyll a fluorescence in research on plants and other photosynthesizing organisms, such as algae and cyanobacteria. This book characterizes the phenomenon of chlorophyll a fluorescence, describes the methods for its measurement, and demonstrates — using selected examples — the applicability of these methods to research the response of the photosynthetic apparatus and plant tolerance to unfavorable environmental conditions. In addition, chapters cover a general background on photosynthesis, analysis of delayed fluorescence, and the pulse amplitude modulated (PAM) technique. The book is addressed to a wide range of professionals in photosynthesis research and scientists from other areas of plant sciences.

Measurements of variable chlorophyll fluorescence have revolutionised global research of photosynthetic bacteria, algae and plants and in turn assessment of the status of aquatic ecosystems, a success that has partly been facilitated by the widespread commercialisation of a suite of chlorophyll fluorometers designed for almost every application in lakes, rivers and oceans. Numerous publications have been produced as researchers and assessors have simultaneously sought to optimise protocols and practices for key organisms or water bodies; however, such parallel efforts have led to difficulties in reconciling processes and patterns across the aquatic sciences. This book follows on from the first international conference on “chlorophyll fluorescence in the aquatic sciences” (AQUAFLUO 2007): to bridge the gaps between the concept, measurement and application of chlorophyll fluorescence through the synthesis and integration of current knowledge from leading researchers and assessors as well as instrument manufacturers.

Chlorophyll a Fluorescence: A Signature of Photosynthesis highlights chlorophyll (Chl) a fluorescence as a convenient, non-invasive, highly sensitive, rapid and quantitative probe of oxygenic photosynthesis. Thirty-one chapters, authored by 58 international experts, provide a solid foundation of the basic theory, as well as of the application of the rich information contained in the Chl a fluorescence signal as it relates to photosynthesis and plant productivity. Although the primary photochemical reactions of photosynthesis are highly efficient, a small fraction of absorbed photons escapes as Chl fluorescence, and this fraction varies with metabolic state, providing a basis for monitoring quantitatively various processes of photosynthesis. The book explains the mechanisms with which plants defend themselves against environmental stresses (excessive light, extreme temperatures, drought, hyper-osmolarity, heavy metals and UV). It also includes discussion on fluorescence imaging of leaves and cells and the remote sensing of Chl fluorescence from terrestrial, airborne, and satellite bases. The book is intended for use by graduate students, beginning researchers and advanced undergraduates in the areas of integrative plant biology, cellular and molecular biology, plant biology, biochemistry, biophysics, plant physiology, global ecology and agriculture.

This book is dedicated to international cooperation, understanding and peace. It is the end result of several years of cooperative work between scientists of three countries: the United States, Germany, and Portugal. The work presented, however, draws from a much broader base, hopefully achieving the objective of NATO Advanced Research Workshops, which have been established to allow and stimulate the exchange of new ideas and the synthesis of information by scientists of NATO countries. The tasks of the workshop were several; to review established methodologies that have provided insight into ecosystem function and adaptations of plants in mediterranean climate zones; to examine new methodologies that have recently been applied in ecological studies and have provided new types of information; to summarize recent studies in mediterranean regions of plant water relations, photosynthesis and production, mineral nutrition, plant growth and development, and response to fire; to stimulate in particular an exchange of information among scientists of European Mediterranean countries; and to discuss means by which all of these objectives might be even more effectively achieved in the future through cooperative international research efforts. This variety of themes is clearly evident in the layout of the book. Held in Sesimbra, Portugal in October of 1985, the workshop took place in a ..

The majority of the world's people depend research work should be carried out at the local and regional level by locally trained on plants for their livelihood since they grow them for food, fuel, timber, fodder and people. many other uses. A good understanding Following the success of our earlier book of the practical factors which govern the (Techniques in Bioproductivity and Photo synthesis; Pergamon Press, 1985), which productivity of plants through the process of photosynthesis is therefore of paramount was translated into four major languages, importance, especially in the light of cur the editors and contributors have exten rent concern about global climate change sively revised the content and widened the and the response of both crops and natural scope of the text,· so it now bears a title ecosystems. in line with current concern over global The origins of this book lie in a series of climate change. · In particular, we have training courses sponsored by the United added chapters on remote sensing, con Nations Environment Programme (Project trolled-environment studies, chlorophyll No. FP/6108-88-0l (2855); 'Environment fluorescence, metabolite partitioning and changes and the productivity of tropical the use of mass isotopes, all of which grasslands'), with additional support from techniques are increasing in their applica many international and national agencies. tion and importance to this subject area.

In a world of increasing atmospheric CO2, there is intensified interest in the ecophysiology of photosynthesis and increasing attention is being given to carbon exchange and storage in natural ecosystems. We need to know how much photosynthesis of terrestrial and aquatic vegetation will change as global CO2 increases. Are there major ecosystems, such as the boreal forests, which may become important sinks of CO2 and slow down the effects of anthropogenic CO2 emissions on climate? Will the composition of the vegetation change as a result of CO2 increase? This volume reviews the progress which has been made in understanding photosynthesis in the past few decades at several levels of integration from the molecular level to canopy, ecosystem and global scales.