Purification And Characterization Of Secondary Metabolites

Purification and Characterization of Secondary Metabolites: A Laboratory Manual for Analytical and Structural Biochemistry provides students with working knowledge of the fundamental and advanced techniques of experimental biochemistry. Sections provide an overview of the microbiological and biochemical methods typically used for the purification of metabolites and discuss the biological significance of secondary metabolites secreted by three diverse species of bacteria. Additionally, this lab manual covers the theory and practice of the most commonly-used techniques of analytical biochemistry, UV-vis and IR spectrophotometry, high-performance liquid chromatography, mass spectrometry, X-ray crystallography and nuclear magnetic resonance, and how to evaluate and effectively use scientific data. Instructors will find this book useful because of the modular nature of the lab exercises included. Written in a logical, easy-to-understand manner, this book is an indispensable resource for both students and instructors. Offers project lab formats for students that closely simulate original research projects Provides instructional guidance for students to design their own experiments Presents advanced analytical techniques Includes access to a website with additional resources for instructors

India is known for its Ayurvedic system of medicine significantly based on therapeutic plants. Medicinal plants are used since time immemorial due to its safety, efficacy, cultural acceptability and lesser side effects as compared to synthetic drugs. In this present book, a scientific approach has been extensively applied for isolation, purification and screening of biological potential based on bioassay-guided fractionation methods. More specifically, the traditional values of therapeutic plants are correlated with scientific approach for the validation of “drug- like properties”. This book is quite helpful for finding the hidden values of therapeutic approach of ethno-medicinal plants. This book is inclusively a soul combination of pharmacognosy, biotechnology, bioinformatics and nanotechnology which are the most thrusting subjects of today’s world. This book is a must-read for science students, research scholars and scientific community who are interested in plant science.

Food security and the medicinal needs of billions of people around the world are pressing global issues, and the biodiversity and sustainable utilization of plants is of great significance in this context. Further, ethnobotanical studies are vital in the discovery of new drugs from indigenous medicinal plants, and plants with industrially important metabolites need to be cultivated to meet the growing market demand. In addition, the production of plant metabolites under in vitro conditions also has tremendous possibilities. The totipotency of plant cells plays a valuable role in the sustainable utilization of plant resources through cell, tissue and organ culture. At the same time, production can be enhanced using productive cell lines, treatment with elicitors, changing nutritional parameters and metabolic engineering. This book provides state-of-the-art information on biodiversity, conservation, ethnobotany, various aspects of In vitro secondary metabolite production, bioprospecting from various plant groups and drug discovery. It also discusses methods of extracting and characterizing drug leads from plant sources.​

Bacterial Secondary Metabolites: Synthesis and Applications in Agroecosystem presents the structure, properties, and biotechnological applications of bacterial metabolites and their upcoming industrial, pharmaceutical, antimicrobial, and anticancer applications. Chapters cover topics such as the use of lactic acid bacteria as an antifungal and antibacterial agent, bacterial siderophores structure and potential applications, and the role of cyanobacteria metabolites in disease management, among others. Plant and agri-food environmental scientists and researchers, graduate and post-graduate students in related fields will benefit from this reference book which is published as part of the series Nanobiotechnology for Plant Protection. Explores how research might lead to the production of new bio-based commercial solutions to tackle global agricultural and human diseases Contains extensive information to understand the intricate processes of cryptic genes and their relationship to the synthesis of bioactive chemicals Provides in-depth insights into microbial biotechnology, namely secondary metabolites

This book consists of an introductory overview of secondary metabolites, which are classified into four main sections: microbial secondary metabolites, plant secondary metabolites, secondary metabolites through tissue culture technique, and regulation of secondary metabolite production. This book provides a comprehensive account on the secondary metabolites of microorganisms, plants, and the production of secondary metabolites through biotechnological approach like the plant tissue culture method. The regulatory mechanisms of secondary metabolite production in plants and the pharmaceutical and other applications of various secondary metabolites are also highlighted. This book is considered as necessary reading for microbiologists, biotechnologists, biochemists, pharmacologists, and botanists who are doing research in secondary metabolites. It should also be useful to MSc students, MPhil and PhD scholars, scientists, and faculty members of various science disciplines.

The secondary metabolites of plants were once considered to be waste products - today, their true value is understood. New methods of separation and structural elucidation, and advances in the investigation of biochemical activities, have increased our understanding of secondary metabolites. Their function as a defense mechanisms offers a great potential for technological gain. Secondary metabolites can be utilized in agriculture to breed stronger crops and in the manufacture of biorational pesticides. They can also be exploited by medicine as theraputic agents. And these are just two of the likely uses. This landmark volume presents articles by an impressive team of experts from leading laboratories. Each chapter considers a current understanding of secondary metabolites in nature and the potential exploitation of those qualities by the biotechnology industry.

Proceedings of an American Chemical Society symposium on Biosynthesis and Metabolism of Secondary Metabolite Natural Products, held April 1991, in Atlanta, Georgia. Bridging the gap between the fields of mechanistic bio-organic chemistry and biotechnology, contributions are in four main areas: antib

Research on microbes plays an essential role in the improvement of biotechnological and biomedical areas. It has turned into a subject of expanding significance as new organisms and their related biomolecules are being characterized for several applications in health and agriculture. Microbial biomolecules confer the ability of microbes to cope with a range of adverse conditions. However, these biomolecules have several advantages over the plant origin, which makes them a suitable target in drug discovery and development. The reasons could be that microbial sources can be genetically engineered to enhance the production of desired natural production by large-scale fermentation. The interaction between microbes and their biotic and abiotic environment is fundamental to numerous processes taking place in the biosphere. The natural environments and hosts of these microorganisms are extremely diverse being reflected by the fact that microbes are widespread and occur in nearly every biological community on Earth. This metabolic versatility makes microbes interesting objects for a range of economically important biotechnological applications. Most of the biotechniques are established but inefficient genetic engineering strategies are still a bottleneck for selected microbe producing industrial scale biomolecules. Therefore, untapped microbial biodiversity and related metablomics, give a noteworthy wellspring of biologicals for the advancement of meds, immunizations, enhanced plants and for other natural applications. The present eBook volume contains articles on microbial secondary metabolites, microbial biosynthetic potential including biosynthetic gene expression, and metagenomics obtained from microorganism isolated unique from habitats like marine sources, endophytes, thermal springs, deserts, etc.