The Chlamydomonas Sourcebook

Dr. Harris has played a major role in the development of this organism as a model system. Her previous version of the Chlamydomonas Sourcebook which published in 1989, has been a classic in the field and is considered required reading for anyone working with this organism. This latest edition has been expanded to include three volumes providing molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism. Methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media, are included. Additions to this volume also include help with common laboratory problems such as contamination, student demonstrations, and properties of particular strains and mutants. This volume is part of a 3-Volume Set (ISBN: 978-0-12-370873-1) and is also sold individually. Expanded revision of gold standard reference Includes latest advances in research, including completion of the genome Provides broad perspective with studies in cell and molecular biology, genetics, plant physiology and related fields Available as part of a 3-Volume Set or sold individually

The Chlamydomonas Sourcebook, 3rd Edition Introduction to Chlamydomonas and Its Laboratory Use (Volume 1) The gold-standard reference?covering the basic biology of the Chlamydomonas alga and techniques for its laboratory analysis Originally published as the standalone Chlamydomonas Sourcebook, then expanded as the first volume in a three-part comprehensive gold-standard reference,?The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use?has been fully revised and updated to include a?wealth of new resources for the Chlamydomonas community. Early chapters cover current understandings of its taxonomy, ultrastructure, cell and life cycles, and nuclear and organelle genomes, followed by technique-oriented chapters covering such topics as cell culture, mutagenesis, genetic analysis, construction of mutant libraries, and protein localization using immunofluorescence. This volume presents the latest in research and best practices, making it a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, and biochemistry; crop scientists; plant physiologists; and plant, molecular, and human disease biologists. Remains the only complete reference to provide both the historical background and the most up-to-date information and applications on Chlamydomonas Includes best practices for applications in research, including methods for culture, genetic analysis, genomic and transcriptomic analysis, and mutant screening Helps researchers solve common laboratory problems, provides details on the properties of particular strains, and offers a comprehensive survey of molecular approaches Provides a broad perspective for studies in cell and molecular biology, genetics, plant physiology, and related fields

The green alga Chlamydomonas is widely used as an experimental model system for studies in cellular and molecular biology, and in particular plant molecular biology. This book is the only single modern compendium of information on its biology and in particular its molecular biology and genetics. Included in addition to much information on the basic biology is material of a very practical nature, namely, methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media, help with common laboratory problems such as contamination, student demonstrations, and properties of particular strains and mutants. Casual users as well as specialists will find the book to be useful in many ways. Key Features * Provides access to previously unpublished data from genetic analysis * Provides descriptions of mutant strains * Depicts summary tables comparing properties of different species and their mutant strains * Explains detailed methods for laboratory procedures of general utility * Furnishes comparisons of culture media * Presents lists of inhibitors, mutagens, and other additives to culture media * Assists with common laboratory problems such as contamination and storage of strains * Demonstrates protocols for laboratory demonstrations available for undergraduate teaching.

The Chlamydomonas Sourcebook, Third Edition, Volume Two: Organellar and Metabolic Processes has been fully revised and updated to include a wealth of new resources for the Chlamydomonas community. Long known for its comprehensive insights into this highly multidisciplinary topic, this edition includes new views on Chlamydomonas branched electron transfer pathways in photosynthesis, the organization of pyrenoids and CO2 concentration mechanisms, and the fermentative-aspects of dark metabolism. Further, the book covers Chlamydomonas and its use in microalgae biotechnology. In addition to updates to previous editions, this book presents the latest in research and best practices, thus making it a must-have resource for those working with the Chlamydomonas organism. Describes molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism Provides methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors, and other additives to culture media Assists researchers with common laboratory problems such as contamination

Originally published as the stand-alone Chlamydomonas Sourcebook, then expanded as the second volume in a three-part comprehensive gold-standard reference, The Chlamydomonas Sourcebook: Organellar and Metabolic Processes has been fully revised and updated to include a wealth of new knowledge and resources for the Chlamydomonas community. It details the tremendous progress recently made with respect to imaging the ultrastructure of cells, dissecting acclimation and biosynthetic responses, and elucidating molecular processes underlying the biology of organelles. In particular, this volume includes exciting new developments in the use of imaging technologies for examining supramolecular organization of the chloroplast, defining mechanisms of branched electron transfer pathways in photosynthesis, dissecting the organization of pyrenoids and CO2 concentration mechanisms, presenting the intricacies associated with acclimation to environmental conditions and providing new insights into dark metabolism and the network of fermentative metabolism. This book thus presents the latest advances in both the research and uses of new experimental approaches and technologies, making this a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, aspects of human disease, acclimation to environmental change, and the biogenesis of cellular complexes. Describes molecular techniques, analysis of the recently sequenced genome, reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism Provides methods for Chlamydomonas research and best practices for their applications; this includes methods for cell culture, preservation of cultures, preparation of media, lists of inhibitors, and other additives to culture media, classical genetic manipulation, and new approaches for gene transfer and editing technologies Assists researchers with common laboratory problems such as contamination

The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use, Volume One, Third Edition has been fully revised and updated to include a wealth of new resources for the Chlamydomonas community (new mutant libraries, new omics studies, and potentially more information about different Chlamydomonas species in the environment). In addition to updates on molecular techniques and analysis of the sequenced genome, the book presents the latest in research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, and more. Provides the only complete reference on both the historical background and most up-to-date information and applications on Chlamydomonas Edited by current leaders in the area, continuing the expert insights established through the first two editions Includes best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors, and more Aids researchers with common laboratory problems such as contamination and details on properties of particular strains and mutants

The Chlamydomonas Sourcebook, 3rd Edition Cell Motility and Behavior (Volume 3) The gold-standard reference?introducing this multidisciplinary science,?fully revised and updated with the latest discoveries Originally published as the standalone Chlamydomonas Sourcebook, then expanded as the third volume in a three-part comprehensive gold-standard reference,?The Chlamydomonas Sourcebook: Cell Motility and Behavior has been fully revised and updated to include the wealth of new resources for the Chlamydomonas community. Reflecting the significant advancement in?the understanding of the role of basal bodies and cilia play in human diseases, this volume employs quantitative proteomics and mass spectroscopy as well as?cryo?EM tomography and single particle?cryo?EM. Other topics such as current insights?on?mitosis and cytokinesis, ciliary assembly and motility, intraflagellar?transport, and more help build an understanding of human diseases of the cilium. Cell Motility and Behavior presents the latest in research and best practices, making this a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, and biochemistry; crop scientists; plant physiologists; and plant, molecular, and human disease biologists. Provides an essential reference to a model species for the study of mechanisms of motility in free living cells? Includes methods for Chlamydomonas motility research? Includes a table listing the known proteins (with NCBI accession numbers) for each structure discussed, and the known mutations that affect each structure and process

This second volume of The Chlamydomonas Sourcebook provides the background and techniques for using this important organism in plant research. From biogenesis of chloroplasts and mitochondria and photosynthesis to respiration and nitrogen assimilation, this volume introduces scientists to the functions of the organism. The volume then moves on to starch biosynthesis, sulfur metabolism, response to heavy metals, and hydrogen production. * Describes molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism * Includes contributions from leaders in particular areas of research * Provides methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media * Assists researchers with common laboratory problems such as contamination * Includes valuable student demonstrations and properties of particular strains and mutants * Edited by the leading researcher in Chlamydomonas science