Wildlife Dna Analysis

Clearly structured throughout, the introduction highlights the different types of crime where these techniques are regularly used. This chapter includes a discussion as to who performs forensic wildlife examinations, the standardisation and validation of methods, and the role of the expert witness in this type of alleged crime. This is followed by a detailed section on the science behind DNA typing including the problems in isolating DNA from trace material and subsequent genetic analysis are also covered. The book then undertakes a comprehensive review of species testing using DNA, including a step-by-step guide to sequence comparisons. A comparison of the different markers used in species testing highlights the criteria for a genetic marker. A full set of case histories illustrates the use of the different markers used. The book details the use of genetic markers to link two or more hairs/feather/leaves/needles to the same individual organism and the software used in population assignment. The problems and possibilities in isolating markers, along with the construction of allele databases are discussed in this chapter. The book concludes with evaluation and reporting of genetic evidence in wildlife forensic science illustrated by examples of witness statements.

The range of species that fall within the realm of wildlife crimes is extensive, ranging from ferns and orchids to bald eagles and great whales. Solving these crimes is rarely dependent on the testimony of witnesses or victims. An ever-increasing number of research groups are applying scientific tests to animal and plant studies alike. However, until now, whatever progress is available in this area has remained scattered through the literature. Forensic Science in Wildlife Investigations focuses on the developing test methods that can be applied to wildlife crimes. In large part, the tests described are drawn from human-based research. Edited by Adrian Linacre, a noted forensic researcher and one of the principal pioneers active in wildlife forensics, this volume collects the work of others working across the world with both plant and animal investigations. While the book contains valuable approaches that lab investigators can employ, the scientific material is written at a level that requires no more than a fundamental knowledge of biology. Any required scientific information is provided in separate boxes. Offering practical guidance, it helps investigators and lab technicians decide on best methods, including a determination of when basic microscopy is sufficient, when DNA testing should occur, and what tests or combination of tests should be executed in a particular circumstance. The text illustrates how to identify the species and geographic region of origin of an unknown sample. Demonstrating the latest methods through real-world case studies, this volume provides the direction and practical advice needed by legal and police professionals seeking to gain the evidence needed to prosecute wildlife crimes.

Wildlife Forensics: Methods and Applications provides an accessible and practical approach to the key areas involved in this developing subject. The book contains case studies throughout the text that take the reader from the field, to the lab analysis to the court room, giving a complete insight into the path of forensic evidence and demonstrating how current techniques can be applied to wildlife forensics. The book contains approaches that wildlife forensic investigators and laboratory technicians can employ in investigations and provides the direction and practical advice required by legal and police professionals seeking to gain the evidence needed to prosecute wildlife crimes. The book will bring together in one text various aspects of wildlife forensics, including statistics, toxicology, pathology, entomology, morphological identification, and DNA analysis. This book will be an invaluable reference and will provide investigators, laboratory technicians and students in forensic Science/conservation biology classes with practical guidance and best methods for criminal investigations applied to wildlife crime. Includes practical techniques that wildlife forensic investigators and laboratory technicians can employ in investigations. Includes case studies to illustrate various key methods and applications. Brings together diverse areas of forensic science and demonstrates their application specifically to the field of wildlife crime. Contains methodology boxes to lead readers through the processes of individual techniques. Takes an applied approach to the subject to appeal to both students of the subject and practitioners in the field. Includes a broad introduction to what is meant by 'wildlife crime', how to approach a crime scene and collect evidence and includes chapters dedicated to the key techniques utilized in wildlife investigations. Includes chapters on wildlife forensic pathology; zooanthropological techniques; biological trace evidence analysis; the importance of bitemark evidence; plant and wildlife forensics; best practices and law enforcement.

Forensic DNA Analysis: Technological Development and Innovative Applications provides a fascinating overview of new and innovative technologies and current applications in forensic genetics. Edited by two forensic experts with many years of forensic crime experience with the Italian police and with prestigious academic universities, the volume takes an interdisciplinary perspective, the volume presents an introduction to genome polymorphisms, discusses, forensic genetic markers, presents a variety of new methods and techniques in forensic genetics, and looks at a selection of new technological innovations and inventions now available from commercial vendors. The book is an important resource for scientists, researchers, and other experts in the field who will find it of interest for its exhaustive discussion of the most important technological innovations in forensic genetics. For those newer to the field, the volume will be an invaluable reference guide to the forensic world.

The book explores the fundamental principles, advances in forensic techniques, and its application on forensic DNA analysis. The book is divided into three modules; the first module provides the historical prospect of forensic DNA typing and introduces fundamentals of forensic DNA typing, methodology, and technical advancements, application of STRs, and DNA databases for forensic DNA profile analysis. Module 2 examines the problems and challenges encountered in extracting DNA and generating DNA profiles. It provides information on the methods and the best practices for DNA isolation from forensic biological samples and human remains like ancient DNA, DNA typing of skeletal remains and disaster victim identification, the importance of DNA typing in human trafficking, and various problems associated with capillary electrophoresis. Module 3 emphasizes various technologies that are based on SNPs, STRs namely Y-STR, X-STR, mitochondrial DNA profiling in forensic science. Module 4 explores the application of non-human forensic DNA typing of domestic animals, wildlife forensics, plant DNA fingerprinting, and microbial forensics. The last module discusses new areas and alternative methods in forensic DNA typing, including Next-Generation Sequencing, and its utility in forensic science, oral microbes, and forensic DNA phenotyping. Given its scope, the book is a useful resource in the field of DNA fingerprinting for scientists, forensic experts, and students at the postgraduate level.

The increasingly arcane world of DNA profiling demands that those needing to understand at least some of it must find a source of reliable and understandable information. Combining material from the successful Wiley Encyclopedia of Forensic Science with newly commissioned and updated material, the Editors have used their own extensive experience in criminal casework across the world to compile an informative guide that will provide knowledge and thought-provoking articles of interest to anyone involved or interested in the use of DNA in the forensic context. Following extensive introductory chapters covering forensic DNA profiling and forensic genetics, this comprehensive volume presents a substantial breadth of material covering: Fundamental material – including sources of DNA, validation, and accreditation Analysis and interpretation – including, extraction, quantification, amplification and interpretation of electropherograms (epgs) Evaluation – including mixtures, low template, and transfer Applications – databases, paternity and kinship, mitochondrial-DNA, wildlife DNA, single-nucleotide polymorphism, phenotyping and familial searching Court - report writing, discovery, cross examination, and current controversies With contributions from leading experts across the whole gamut of forensic science, this volume is intended to be authoritative but not authoritarian, informative but comprehensible, and comprehensive but concise. It will prove to be a valuable addition, and useful resource, for scientists, lawyers, teachers, criminologists, and judges.

DNA Analysis has been an important tool for forensic investigations, used to scientifically link an individual to a crime, and provide statistical relevance to the possibility that any other person could have contributed that biological evidence. This analysis has predominantly focused on human biological evidence, but animal-sourced evidence can provide equally compelling information to an investigation. Such analysis could be useful in cases that involve trace transfer of animal hairs (such as from a pet of the perpetrator onto a victim), cases that involve an animal directly (such as a animal cruelty cases), or cases that involve wildlife (such as the trade of endangered animal parts). Studies show that ~50% of US households contain a dog or cat, and that wildlife trade is estimated at $20 billion a year. However, animal DNA evidence has been used in only a small number of courtroom cases. This research seeks to outline the potential, limitations, and necessary development in research and technology to apply DNA analysis to animal evidence.

The association of a suspect with the victim or crime scene through DNA evidence is one of the most powerful statements of complicity in a crime imaginable. No category of evidence has ever had the complete capacity to convict or exonerate an accused so absolutely in the eyes of the public. With the discriminatory powers of DNA and the variety of D