Methods In Plant Biochemistry And Molecular Biology

Modern plant science research currently integrates biochemistry and molecular biology. This book highlights recent trends in plant biotechnology and molecular genetics, serving as a working manual for scientists in academic, industrial, and federal laboratories. A wide variety of authors have contributed to this book, reflecting the thinking and expertise of active investigators who generate advances in technology. The authors were selected especially for their ability to create and/or implement novel research methods.

Book extensively deals with the Plant Sciences and the experiments can be easily tailored to suit individual conditions, hence should be of general interest to researchers and teachers who frame the syllabi. PREFACE.

Modern plant science research currently integrates biochemistry and molecular biology. This book highlights recent trends in plant biotechnology and molecular genetics, serving as a working manual for scientists in academic, industrial, and federal laboratories. A wide variety of authors have contributed to this book, reflecting the thinking and expertise of active investigators who generate advances in technology. The authors were selected especially for their ability to create and/or implement novel research methods.

Methods in Plant Biochemistry, Volume 1: Plant Phenolics reviews current knowledge about techniques used in the analysis of the biochemistry of plant polyphenols and their importance in the agricultural and food industries. It looks at the application of these techniques in the fractionation of cellular constituents, isolation of enzymes, electrophoretic separation of nucleic acids and proteins, and chromatographic identification of the intermediates and products of cellular metabolism. Organized into 15 chapters, this book opens with an overview of the general procedures and measurement of total phenolics, from detecting phenolic substances in crude plant extracts to determining which classes they belong to and the quantitative estimation of total phenol. The reader is introduced to the chemistry, structural variation, function, and distribution of each class of plant phenolics and, in a few cases where this is practicable, detailed listings of known derivatives are given. Most chapters focus on chromatographic separations and high performance liquid chromatography (HPLC), along with thin layer and paper Rf values with HPLC retention times and NMR spectroscopy. The book also outlines the procedures for the extraction, isolation, separation, and characterization of different classes of phenolic compounds, ranging from phenols and phenolic acids to phenylpropanoids, lignins, stilbenes and phenanthrenes, flavones and flavonols, chalcones and aurones, flavanoids, anthocyanins, biflavanoids, tannins, isoflavanoids, quinones, xanthones, and lichen substances. The book is a valuable resource for students, biochemists, and researchers in the plant sciences.

This series, Methods in Plant Biochemistry, provides a reference on current techniques in the various fields of plant biochemical research. Under the guidance of a guest editor, each volume provides comprehensive, practical information on the assay and analytical technqiues appropriate for a particular family of plant compounds. The emphasis here is on techniques and laboratory procedure.

The fully revised and expanded fourth edition of Plant Biochemistry presents the latest science on the molecular mechanisms of plant life. The book not only covers the basic principles of plant biology, such as photosynthesis, primary and secondary metabolism, the function of phytohormones, plant genetics, and plant biotechnology, but it also addresses the various commercial applications of plant biochemistry. Plant biochemistry is not only an important field of basic science explaining the molecular function of a plant, but is also an applied science that is in the position to contribute to the solution of agricultural and pharmaceutical problems. Plants are the source of important industrial raw material such as fat and starch but they are also the basis for the production of pharmaceutics. It is expected that in the future, gene technology will lead to the extensive use of plants as a means of producing sustainable raw material for industrial purposes. As such, the techniques and use of genetic engineering to improve crop plants and to provide sustainable raw materials for the chemical and pharmaceutical industries are described in this edition. The latest research findings have been included, and areas of future research are identified. Offers the latest research findings in a concise and understandable manner Presents plant metabolism in the context of the structure and the function of plants Includes more than 300 two-color diagrams and metabolic schemes Covers the various commercial applications of plant biochemistry Provides extensive references to the recent scientific literature