Methods In Plant Biochemistry

Crown gall tumours on potato discs and brine shrimp lethality: two simple bioassays for higher plant screening and fractionation. Assays for antifungal activity. Screening methods for antibacterial and antiviral agents from higher plants. Assays related to cancer drug discovery. Assays for antimalarial and amoebicidal activities. Assays for molluscicidal, cercaricidal and piscicidal activities. Screening techniques for plant-insect interactions. Assay methods for antihepatotoxic activity. Assays for pharmacological activity. A primer in plant electrophysiological methods. Organ culture and manipulation.

Methods in Plant Biochemistry, Volume 1: Plant Phenolics reviews current knowledge about techniques used in the analysis of the biochemistry of plant polyphenols and their importance in the agricultural and food industries. It looks at the application of these techniques in the fractionation of cellular constituents, isolation of enzymes, electrophoretic separation of nucleic acids and proteins, and chromatographic identification of the intermediates and products of cellular metabolism. Organized into 15 chapters, this book opens with an overview of the general procedures and measurement of total phenolics, from detecting phenolic substances in crude plant extracts to determining which classes they belong to and the quantitative estimation of total phenol. The reader is introduced to the chemistry, structural variation, function, and distribution of each class of plant phenolics and, in a few cases where this is practicable, detailed listings of known derivatives are given. Most chapters focus on chromatographic separations and high performance liquid chromatography (HPLC), along with thin layer and paper Rf values with HPLC retention times and NMR spectroscopy. The book also outlines the procedures for the extraction, isolation, separation, and characterization of different classes of phenolic compounds, ranging from phenols and phenolic acids to phenylpropanoids, lignins, stilbenes and phenanthrenes, flavones and flavonols, chalcones and aurones, flavanoids, anthocyanins, biflavanoids, tannins, isoflavanoids, quinones, xanthones, and lichen substances. The book is a valuable resource for students, biochemists, and researchers in the plant sciences.

Methods in Plant Biochemistry, Volume 1: Plant Phenolics reviews current knowledge about techniques used in the analysis of the biochemistry of plant polyphenols and their importance in the agricultural and food industries. It looks at the application of these techniques in the fractionation of cellular constituents, isolation of enzymes, electrophoretic separation of nucleic acids and proteins, and chromatographic identification of the intermediates and products of cellular metabolism. Organized into 15 chapters, this book opens with an overview of the general procedures and measurement of total phenolics, from detecting phenolic substances in crude plant extracts to determining which classes they belong to and the quantitative estimation of total phenol. The reader is introduced to the chemistry, structural variation, function, and distribution of each class of plant phenolics and, in a few cases where this is practicable, detailed listings of known derivatives are given. Most chapters focus on chromatographic separations and high performance liquid chromatography (HPLC), along with thin layer and paper Rf values with HPLC retention times and NMR spectroscopy. The book also outlines the procedures for the extraction, isolation, separation, and characterization of different classes of phenolic compounds, ranging from phenols and phenolic acids to phenylpropanoids, lignins, stilbenes and phenanthrenes, flavones and flavonols, chalcones and aurones, flavanoids, anthocyanins, biflavanoids, tannins, isoflavanoids, quinones, xanthones, and lichen substances. The book is a valuable resource for students, biochemists, and researchers in the plant sciences.

V.1 - Plant phenolics: General procedures and measurement of total phenolics: Phenols and phenolic acids; Phenylpropanoids; Lignins; Stilbenes and phenanthrenes; Flavones, flavonols and their glycosides; Chalcones and aurones; Flavonoids; Anthocyanins; Biflavonoids; Tannins; Isoflavonoids; Quinones; Xanthones; Lichen substances. v.2 - carbohydrates: Monosaccharides; Nucleotide sugars; Lipid-linked saccharides in plant: intermediates in the synthesis of N-linked glycoproteins; Disaccharides; Oligosaccharides; Cyclitols; Branched-chain sugars and sugar alcohols; Cellulose; Starch; Fructans; Mannose-based polysaccharides; The pectic polysacchareides of primary cells walls; Chitin; Exudate gums; Algal polysaccharides; Isolation and analysis of plant cell walls; Anhydrous hydrogen fluoride in Polysaccharide solvolysis and glycoprotein delycosylation; Techniques for studying interactions between polysaccharides. v.3 - Enzymes of primary metabolism: Ribulose bisphosphate carboxylase/oxygenase and carbonic anhydrase; Enzymes of the calvin cycle; Enzymes of C4 photosynthesis; Enzymes of sucrose metabolism; Fructose 2,6-bisphosphate; Enzymes of starch synthesis; Starch degrading enzymes; Enzymes of the photorespiratory carbon pathway; Glycolysis; The mitochondrial pyruvate dehydrogenase complex; Enzymes of fatty acid synthesis; Enzymes of lipid degradation; Enzymes of phospholipid synthesis; Nitrate reductase and nitrite reductase; Enzymes of asparagine metabolism; Enzymes of lysine synthesis; Threonine biosynthesis; Enzymes of leucine, valine and isoleucine biosynthesis; Sulphur metabolism; Adenosine 5'-phosphosulphate sulphotransferase; Sulphite reductase; Cysteine synthase; Synthesis of glutathione; Enzymes involved in the synthesis of methionine; Protein kinase; Tonoplast adenosine triphosphatase and inorganic pyrophosphatase.

Modern plant science research currently integrates biochemistry and molecular biology. This book highlights recent trends in plant biotechnology and molecular genetics, serving as a working manual for scientists in academic, industrial, and federal laboratories. A wide variety of authors have contributed to this book, reflecting the thinking and expertise of active investigators who generate advances in technology. The authors were selected especially for their ability to create and/or implement novel research methods.

The series Methods in Plant Biochemistry provides an authoritative reference on current techniques in the various fields of plant biochemical research. Each volume in the series will, under the expert guidance of a guest editor, deal with a particular group of plant compounds. The historical background and current, most useful methods of analysis are described. Detailed discussions of the protocols and suitability of each technique are included. Case treatments, diagrams, chemical structures, reference data, and properties will be featured along with a full list of references to the specialist literature.**Conceived as a practical comparison to The Biochemistry of Plants, edited by P.K. Stumpf and E.E. Conn, no plant biochemical laboratory can afford to be without this comprehensive and up-to-date reference source.

The series Methods in Plant Biochemistry provides an authoritative reference on current techniques in the various fields of plant biochemical research. Each volume in the series will, under the expert guidance of a guest editor, deal with a particular group of plant compounds. The historical background and current, most useful methods of analysis are described. Detailed discussions of the protocols and suitability of each technique are included. Case treatments, diagrams, chemical structures, reference data, and properties will be featured along with a full list of references to the specialist literature.**Conceived as a practical comparison to The Biochemistry of Plants, edited by P.K. Stumpf and E.E. Conn, no plant biochemical laboratory can afford to be without this comprehensive and up-to-date reference source.

Methods in Plant Biochemistry, Volume 1: Plant Phenolics reviews current knowledge about techniques used in the analysis of the biochemistry of plant polyphenols and their importance in the agricultural and food industries. It looks at the application of these techniques in the fractionation of cellular constituents, isolation of enzymes, electrophoretic separation of nucleic acids and proteins, and chromatographic identification of the intermediates and products of cellular metabolism. Organized into 15 chapters, this book opens with an overview of the general procedures and measurement of total phenolics, from detecting phenolic substances in crude plant extracts to determining which classes they belong to and the quantitative estimation of total phenol. The reader is introduced to the chemistry, structural variation, function, and distribution of each class of plant phenolics and, in a few cases where this is practicable, detailed listings of known derivatives are given. Most chapters focus on chromatographic separations and high performance liquid chromatography (HPLC), along with thin layer and paper Rf values with HPLC retention times and NMR spectroscopy. The book also outlines the procedures for the extraction, isolation, separation, and characterization of different classes of phenolic compounds, ranging from phenols and phenolic acids to phenylpropanoids, lignins, stilbenes and phenanthrenes, flavones and flavonols, chalcones and aurones, flavanoids, anthocyanins, biflavanoids, tannins, isoflavanoids, quinones, xanthones, and lichen substances. The book is a valuable resource for students, biochemists, and researchers in the plant sciences.