Methods In Plant Biochemistry Terpenoids

V.1 - Plant phenolics: General procedures and measurement of total phenolics: Phenols and phenolic acids; Phenylpropanoids; Lignins; Stilbenes and phenanthrenes; Flavones, flavonols and their glycosides; Chalcones and aurones; Flavonoids; Anthocyanins; Biflavonoids; Tannins; Isoflavonoids; Quinones; Xanthones; Lichen substances. v.2 - carbohydrates: Monosaccharides; Nucleotide sugars; Lipid-linked saccharides in plant: intermediates in the synthesis of N-linked glycoproteins; Disaccharides; Oligosaccharides; Cyclitols; Branched-chain sugars and sugar alcohols; Cellulose; Starch; Fructans; Mannose-based polysaccharides; The pectic polysacchareides of primary cells walls; Chitin; Exudate gums; Algal polysaccharides; Isolation and analysis of plant cell walls; Anhydrous hydrogen fluoride in Polysaccharide solvolysis and glycoprotein delycosylation; Techniques for studying interactions between polysaccharides. v.3 - Enzymes of primary metabolism: Ribulose bisphosphate carboxylase/oxygenase and carbonic anhydrase; Enzymes of the calvin cycle; Enzymes of C4 photosynthesis; Enzymes of sucrose metabolism; Fructose 2,6-bisphosphate; Enzymes of starch synthesis; Starch degrading enzymes; Enzymes of the photorespiratory carbon pathway; Glycolysis; The mitochondrial pyruvate dehydrogenase complex; Enzymes of fatty acid synthesis; Enzymes of lipid degradation; Enzymes of phospholipid synthesis; Nitrate reductase and nitrite reductase; Enzymes of asparagine metabolism; Enzymes of lysine synthesis; Threonine biosynthesis; Enzymes of leucine, valine and isoleucine biosynthesis; Sulphur metabolism; Adenosine 5'-phosphosulphate sulphotransferase; Sulphite reductase; Cysteine synthase; Synthesis of glutathione; Enzymes involved in the synthesis of methionine; Protein kinase; Tonoplast adenosine triphosphatase and inorganic pyrophosphatase.

The series, Methods in Plant Biochemistry, provides an authoritative reference on current techniques in the various fields of plant biochemical research. Each volume in the series will, under the expert guidance of a guest editor, deal with a particular group of plant compounds. Each will describe the historical background and current, most useful methods of analysis. The volumes include detailed discussions of the protocols and suitability of each technique. Case treatments, diagrams, chemical structures, reference data, and properties will be featured along with a full list of references to the specialist literature. Conceived as a practical companion to The Biochemistry of Plants, edited by P.K. Stumpf and E.E. Conn, no plant biochemical laboratory can afford to be without this comprehensive and up-to-date reference source. Each volume in the series deals with the analysis of a group of plant compounds Contains authoritative and detailed practical instructions and recipes for analytical techniques

Recent Advances in Phytochemistry, Volume 6: Terpenoids: Structure, Biogenesis, and Distribution covers the advances in the chemistry and biochemistry of terpenoids, and the use of information regarding the occurrence of such compounds in genetics and population ecology. The book discusses the applications of physical methods to some structural and stereochemical problems in terpenes and steroids; novel sesquiterpenes isolated in composites; and the chemistry and biogenesis of the quassinoids (Simaroubolides). The text then describes the recent developments in the biosynthesis of plant triterpenes; the mechanisms of indole alkaloid biosynthesis, recognition of intermediacy and sequence by short-term incubation; and the biochemistry and physiology of lower terpenoids. The genetic and biosynthetic relationships of monoterpenes; and the confirmation of a clinal pattern of chemical differentiation in Juniperus virginiana from terpenoid data obtained in successive years are also encompassed. Botanists, biochemists, and people involved in the study of phytochemistry will find the book invaluable.

The plant terpenoids exhibit enormous structural and functional diversity. They are involved in regulating plant growth, acting as accessory pigments in photosynthesis, and attracting animals to plants for pollination purposes. This is the most up-to-date work on the subject, authored by a panel of distinguished international experts. It reviews the chemistry, chemistry, chemotaxonomy, biochemistry, biosynthesis, physiology, pharmacology, and toxicology of plant terpenoids. The work will interest plant and ecological biochemists, and plant scientists.

Modern plant science research currently integrates biochemistry and molecular biology. This book highlights recent trends in plant biotechnology and molecular genetics, serving as a working manual for scientists in academic, industrial, and federal laboratories. A wide variety of authors have contributed to this book, reflecting the thinking and expertise of active investigators who generate advances in technology. The authors were selected especially for their ability to create and/or implement novel research methods.

This long awaited third edition of Phytochemical Methods is, as its predecessors, a key tool for undergraduates, research workers in plant biochemistry, plant taxonomists and any researchers in related areas where the analysis of organic plant components is key to their investigations. Phytochemistry is a rapidly expanding area with new techniques being developed and existing ones perfected and made easier to incorporate as standard methods in the laboratory. This latest edition includes descriptions of the most up-to-date methods such as HPLC and the increasingly sophisticated NMR and related spectral techniques. Other methods described are the use of NMR to locate substances within the plant cell and the chiral separation of essential oils. After an introductory chapter on methods of plant analysis, individual chapters describe methods of identifying the different type of plant molecules: phenolic compounds, terpenoids, organic acids, lipids and related compounds, nitrogen compounds, sugar and derivatives and macromolecules. Different methods are discussed and recommended, and guidance provided for the analysis of compounds of special physiological relevance such as endogenous growth regulators, substances of pharmacological interest and screening methods for the detection of substances for taxonomic purposes. It also includes an important bibliographic guide to specialized texts. This comprehensive book constitutes a unique and indispensable practical guide for any phytochemistry or related laboratory, and provides hands-on description of experimental techniques so that students and researchers can become familiar with these invaluable methods.