Recent Developments In Embryo Transfer

Embryo transfer is one of the steps in the entire procedure of assisted reproduction. One of the most prolific high-end enterprises today is the embryo transfer domain. This book documents the recent advances and outlooks on newly postulated theories and technological advances related to the transfer of human embryo. The areas of special emphasis discuss challenges faced during the procedure. A few instances of how to improve pregnancy rate by novel techniques have been listed in this book, to give interested readers like embryologists and human IVF specialists, current and useful knowledge, including some critical practice techniques. Detailed contents shed light on the optimal stimulus systems for ovaries, advancements in insemination procedures, advanced embryo transfer technologies and endometrial receptivity, and embryo implantation mechanism. Hence, this book will vastly add to the current knowledge of readers on improving human embryo transfer pregnancy rate.

The continued debate regarding the stage at which the human embryo conceived in the laboratory should be placed in the mother, combined with recent developments in culture media formulations, have brought the role of the human blastocyst in ART back into the spotlight. ART and the Human Blastocyst presents the proceedings of the International Symposium on ART and the Human Blastocyst held from March 30- April 2, 2000 in Dana Point, California. This book brings to the forefront the main issues raised with the transfer of embryos at the blastocyst stage, including the reduction of high order multiple gestations and the role of the blastocyst culture and transfer in facilitating successful single embryo transfer. Sections include gamete quality and pregnancy outcome, physiology of the embryo, blastocyst development in culture, blastocyst transfer and fate, and implantation. More than 40 illustrations and 25 tables complement the text.

Embryo transfer has become one of the prominent high businesses worldwide. This book updates and reviews some new developed theories and technologies in the human embryo transfer and mainly focus on discussing some encountered problems during embryo transfer, which gives some examples how to improve pregnancy rate by innovated techniques so that readers, especially embryologists and physicians for human IVF programs, may acquire some new and usable information as well as some key practice techniques. Major contents include the optimal stimulation scheme for ovaries, advance in insemination technology, improved embryo transfer technology and endometrial receptivity and embryo implantation mechanism. Thus, this book will greatly add new information for readers to improve human embryo transfer pregnancy rate.

3000 new references added since the first editionGives information necessary to produce embryos totally through in vitro techniques Shows commercial applications of embryo and oocyte researchCattle remain at the forefront of many new developments in reproductive technology and what can be done for the cow today will later be applicable to other farm livestock and perhaps humans. This new edition reviews the considerable advances and issues in embryo production technology, based on reports since the first edition in 1994. This is a must have volume for those who own the first edition, and in itself an incredibly informative text.

The use of human in vitro fertilization in the management of infertility is the outgrowth of years of laboratory observations on in vitro sperm-egg interaction. "The editors of this work have themselves contributed significantly to basic knowledge of the mammalian fertilization process. The observations of Don Wolf on sperm penetration, the block to polyspermy and, most recently, sperm hyperactivation in the monkey and human, Gregory Kopf's elucidation of the mechanisms of sperm activation during penetration and the reciprocal dialogue between sperm and egg, and Barry Bavister's definition of culture conditions and requirements necessary for in vitro oocyte maturation, fertilization and development in model mammalian systems including nonhuman primates have contributed greatly to our understanding of the mammalian fertilization process. Wolf, Kopf and Gerrity have enjoyed substantial interaction with clinicians in Departments of Obstetrics and Gynecology and have been directly involved with successful IVF programs. Both Wolf and Kopf have served as research scientists in the Division of Reproductive Biology at the University of Pennsylvania, which, for more than 22 years, has fostered co-mingling of clinically oriented and basic science faculty. It is through such interaction, which clearly exists at many institutions including the University of Wisconsin, that the process of technology transfer is best served. Without an exquisitely coordinated laboratory, there can be no consistent success in human in vitro fertilization. Quality control is pivotal, but close collaboration between the laboratory and the clinic is also essential as information is shared and correlated.

The births of more than 100 apparently normal infants at term following pregnancies initiated by in vitro fertilization and embryo transfer testifies to the successful clinical application of insights obtained from studies in reproductive biology over the last 20 years. In women, these studies have included: 1) characterization of the changes in blood hormone profiles throughout ovulatory menstrual cycles; 2) documentation of the hormonal composition of antral fluid in developing and degenerating preovulatory follicles; 3) correlation of these observations with the state of oocyte maturation and the fertilizability of the oocyte; 4) application of pharmacologic agents for perturbing the normal hormone profiles to regulate the number of preovulatory follicles developing and the time of ovulation; and 5) development of non-invasi ve methods for monitoring follicular development. Optimizing methods for maturing and fertilizing eggs, for moni toring normal development in vi tro, and for transferring and achieving implantation of embryos are continuing concerns of physicians and scientists responsible for extant programs. In addition, all serious students of reproductive biology should critically examine every facet of the processes which must concatenate to assure birth of normal infants following pregnancies achieved by these methods. However, the literature which contains the informational substrate is dispersed widely in a plethora of journals not always readily accessible. Bringing the essentials together facilitates both rapid retrieval of data and access to relevant literature.

Now in its revised and expanded second edition - including over 20 new chapters - this comprehensive textbook remains a unique and accessible description of the current and developing diagnostic and treatment techniques and technologies comprising in vitro fertilization (IVF). Arranged thematically in sections, each chapter covers a key topic in IVF in a sensible presentation. Parts one and two describe the planning, design and organization of an ART unit and IVF laboratory and equipment and systems, respectively. The sections that follow provide detailed descriptions of IVF techniques, embryo culture methods, sperm processing and selection, insemination procedures, micromanipulation, embryo evaluation, cryopreservation, and embryo transfer. Concluding sections address issues of management and regulation of ART labs across the globe, as well as special topics and emerging techniques and devices. Chapter authors, all experts in the field, contribute their expertise from around the world. With the addition of learning key points and review questions at the beginning and end of each chapter, this new edition of In Vitro Fertilization is a readily accessible, high quality instructional resource for reproductive medicine trainees at all levels. Practicing reproductive endocrinologists, urologists, and embryologists also will find value in the book, as will infertility researchers.

Gamete maturation. Towards a molecular understanding of meiosis in domestic animals. Biology of oocyte maturation. Post-meiotic sperm development in mammals. Molecular probes for testing bovine sperm quality. Fertilization and embryonic development. Fertilization by assisted micromanipulation of gametes. In vitro fertilization in farm animals. Culture of embryos of farm animals. Maternal control of early embryonic development. Inter - and extraspecies pregnancies in equids. Embryonic manipulation and genetic engineering. Gender preselection in mammals. Embryo multiplication in livestock. Present procedures and the potential for improvement. Gene transfer in farm animals. Mechanism of sperm - DNA interaction. Spermatozoa as vectors for introducing exogenous DNA into eggs. Embryonic stem cells from domestic animals. Establishment and potential applications. New trends in embryo transfer. Embryo transfer and new technology in sheep reproduction. Embryo production in vivo: problems and prospects. Embryotechnology in dairy cattle breeding. Practical application of two advanced biotechnologies to bovine embryo transfer: splitting and sexing. Theoretical and practical aspects of equine embryo transfer. Embryo technology in pets and endangered species. Reproductive biotechnology in deer farming. Vitrification of oocytes and embryos. International regulations. Tentative prospective and health regulations of the embryo transfer industry.