The Early Development Of Xenopus Laevis

Amphibian embryos are supremely valuable in studies of early vertebrate development because they are large, handle easily, and can be obtained at many interesting stages. And of all the amphibians available for study, the most valuable is Xenopus laevis,which is easy to keep and ovulates at any time of year in response to simple hormone injections. Xenopusembryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopusdevelopment has emerged, which ensures the species’ continued prominent position among the organisms favored for biological investigation. This manual contains a comprehensive collection of protocols for the study of early development in Xenopusembryos. It is written by several of the field’s most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991. As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere. This volume is essential reading for all investigators interested in the developmental and cell biology of Xenopusand vertebrates generally. Many of the techniques described here are illustrated in an accompanying set of videotapeswhich are cross-referenced to the appropriate section of the manual.

本书的实验方案涵盖了爪蟾胚胎、成体的形态发生,爪蟾中基因表达操作,胚胎的获取和实验常规操作等内容。

Frogs from the genus Xenopus have long been used as model organisms in basic and biomedical research. These frogs have helped unlock key fundamental developmental and cellular processes that have led to important scientific breakthroughs and have had practical application in embryology, cancer research and regenerative medicine. Xenopus Development is a vital resource on the biology and development of these key model organisms, and will be a great tool to researchers using these frogs in various disciplines of biological science. Xenopus Development is divided into four sections, the first three highlight key processes in Xenopus development from embryo to metamophosis. These sections focus on the cellular processes, organogenesis and embryo development. The final section highlights novel techniques and approaches being used in Xenopus research. Providing thorough and detailed coverage, Xenopus Development, will be a timely and welcome volume for those working in cell and molecular biology, genetics, developmental biology and biomedical research. Provides broad overview of the developmental biology of both Xenopus laevis and Xenopus tropicalis Explores cellular to systems development in key biomedical model organisms Timely synthesis of the field of Xenopus biology Highlights key biomedical and basic biological findings unlocked by Xenopus

Frogs from the genus Xenopus have long been used as modelorganisms in basic and biomedical research. These frogs have helpedunlock key fundamental developmental and cellular processes thathave led to important scientific breakthroughs and have hadpractical application in embryology, cancer research andregenerative medicine. Xenopus Development is a vitalresource on the biology and development of these key modelorganisms, and will be a great tool to researchers using thesefrogs in various disciplines of biological science. Xenopus Development is divided into four sections, thefirst three highlight key processes in Xenopus development fromembryo to metamophosis. These sections focus on the cellularprocesses, organogenesis and embryo development. The final sectionhighlights novel techniques and approaches being used in Xenopusresearch. Providing thorough and detailed coverage, XenopusDevelopment, will be a timely and welcome volume for thoseworking in cell and molecular biology, genetics, developmentalbiology and biomedical research. Provides broad overview of the developmental biology of bothXenopus laevis and Xenopus tropicalis Explores cellular to systems development in key biomedicalmodel organisms Timely synthesis of the field of Xenopus biology Highlights key biomedical and basic biological findingsunlocked by Xenopus

This book is the culmination of an international collaboration of zoologists to describe the embryology of Xenopus, including the origins of the organ systems, with the purpose of promoting the use of Xenopus as a model amphibian for embryological study.