Laboratory Guide To Insect Pathogens And Parasites

After the publication of the Diagnostic Manual for the Identification of Insect Pathogens, the authors received many queries asking why they had not included the larger metazoan parasites as well as the microbial forms. An examination of the literature indicated that pictorial guides to the identification of nematodes and the immature stages of insect parasites were unavailable. Consequently we decided to rewrite the sections cover ing insect pathogens and combine these with new sections on ento mogenous nematodes and the immature stages of insect parasites. The result is the present laboratory guide, which is unique in covering all types of biotic agents which are found inside insects and cause them injury or disease. Included as parasites are insects and nematodes. Among the pathogens included are viruses, rickettsias, bacteria, fungi, and protozoans. Emphasis is placed on identification with an attempt to use the most easily recognizable characters. Use of a certain number of technical terms is unavoidable, and explanations of these can be found in most biological dictionaries or the glossary of invertebrate pathology prepared by Steinhaus and Martignoni (1970).

Insect Pathology is designed for a broad spectrum of readers. Is should be useful to students, lecturers, and researchers requiring information about the principles in insect pathology and the biology of pathogens. It should serve as a resource for specialists to learn about other insect pathogen systems, for generalists to become aware of advances in insect pathology, and for scientists and students, beginning or otherwise, interested in learning about insect pathology. This book was originally intended to update the 1949 test by E. A. Steinhaus entitled Principles of Insect Pathology. The purpose for this book was twofold: To serve (1) as a text for an insect pathology and/or biological control class and (2) as a comprehensive reference source. Because this book summarizes much of the available information, its usefulness as a textbook for an insect pathology class is apparent. Although the literature citations are extensive, they are far from complete. The literature in insect pathology is voluminous and for the past decade has been expanding at an almost exponential rate. A complete review of the literature is beyond the scope of the book, and an omission of a reference does not preclude its importance. Our citations, however, should serve as a good starting point for those who wish to obtain further information. We have attempted to cover equally all subdisciplines, but shortcomings are unavoidable. For these, we take full responsibility.

Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers. In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series. The books will be of value to advances researches and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work. This manual describes the broad array of techniques that are used in insect pathology. It will provide biologists, insect pathologists, entomologists, and those interested in biological control, with the necessary information to work on a variety of pathogen groups. This book will be an essential laboratory reference for insect pathologists. Features include: * Step by-step instructions on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens * Details of the practical knowledge needed by beginners to apply the techniques * Chapters written by an international group of experts * Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques * Provides extensive supplemental literature and recipes for media, fixatives and stains

This manual was prepared for the diagnosis of insect diseases caused by infectious agents. The agents (or pathogens) included here are fungi, protozoans, bacteria, viruses, and rickettsias. The present work was prepared after much deliberation and discussion with students and teachers who felt a guide of this type would be valuable for diagnosing the microbial diseases of insects. It was modeled after a seminar given on the same subject at Berke ley, which had as its major goal the recognition and identification of insect pathogens for practical purposes. The present work in cludes numerous timesaving "short cuts" which were developed after years of experience of diagnosing insect diseases. Although emphasis is placed on identification, general back ground information on the various pathogens is also included. Thus, under each of the five groups of pathogens, the following topics are discussed: (1) various types of associations with insects, (2) defini tion and classification, (3) general life cycle, (4) characteristics of diseased insects, (5) factors affecting natural infections, (6) methods of examination, (7) isolation and cultivation, (8) important taxonomic characters, (9) tests for infectivity, (10) storage, (11) an illustrated key to the genera (or group in the case of viruses), and (12) literature, especially that pertaining to identification. Although often included with insect pathogens, entomogenous nematodes are not covered here since illustrated keys to those gen era that infect insects are already available (Poinar, 1975, 1977).

This two-volume edited book highlights and reviews the potential of the fossil record to calibrate the origin and evolution of parasitism, and the techniques to understand the development of parasite-host associations and their relationships with environmental and ecological changes. The book deploys a broad and comprehensive approach, aimed at understanding the origins and developments of various parasite groups, in order to provide a wider evolutionary picture of parasitism as part of biodiversity. This is in contrast to most contributions by parasitologists in the literature that focus on circular lines of evidence, such as extrapolating from current host associations or distributions, to estimate constraints on the timing of the origin and evolution of various parasite groups. This approach is narrow and fails to provide the wider evolutionary picture of parasitism on, and as part of, biodiversity. Volume one focuses on identifying parasitism in the fossil record, and sheds light on the distribution and ecological importance of parasite-host interactions over time. In order to better understand the evolutionary history of parasites and their relationship with changes in the environment, emphasis is given to viruses, bacteria, protists and multicellular eukaryotes as parasites. Particular attention is given to fungi and metazoans such as bivalves, cnidarians, crustaceans, gastropods, helminths, insects, mites and ticks as parasites. Researchers, specifically evolutionary (paleo)biologists and parasitologists, interested in the evolutionary history of parasite-host interactions as well as students studying parasitism will find this book appealing.

Volume I tells you how to perform bioassays for plant and insect hormones, regulators, and pathogens. Moreover, it presents extensive tables that provide the information you need to select the most appropriate bioassay for a particular plant growth regulator or hormone. In addition to the chapters on bioassays, Volume I provides a solid introduction to the theory and practice of natural pesticide use, including in-depth discussions of integrated management systems for weed and pest control,k the state-oof-the-oart use of computers in pest management, and allelo-chemicals as natural protection. Guidelines on toxicological testing and EPA regulation of natural pesticides are also detailed.

Insect Pathology, 2nd ed. Fernando E. Vega and Harry K. Kaya, Editors Foreword May Berenbaum Chapter 1. Scope and Basic Principles of Insect Pathology Harry K. Kaya and Fernando E. Vega Chapter 2 - History of Insect Pathology Elizabeth W. Davidson Chapter 3 - Principles of Epizootiology and Microbial Control David I. Shapiro-Ilan, Denny Bruck, and Lawrence A. Lacey Chapter 4 - Baculoviruses and Other Occluded Insect Viruses Robert Harrison and Kelli Hoover Chapter 5 - RNA Viruses Infecting Pest Insects Yan Ping Chen, James J. Becnel, and Steven M. Valles Chapter 6 - Fungal Entomopathogens Fernando E. Vega, Nicolai V. Meyling, Janet Jennifer Luangsa-ard, and Meredith Blackwell Chapter 7 - Microsporidian Entomopathogens Leellen F. Solter, James J. Becnel, and David H. Oi Chapter 8 - Bacterial Entomopathogens Juan Luis Jurat-Fuentes and Trevor Jackson Chapter 9 - Wolbachia Infections in Arthropod Hosts Grant Hughes and Jason L. Rasgon Chapter 10 - Protistan Entomopathogens Carlos E. Lange ...